To assess the quality of the preserved collagen, the sample is demineralized in dilute/cold acid to eliminate all apatite and carbonate after physically or chemically removing the outer exposed surfaces.
Thus, it’s possible that the bones have very limited collagen.Any rootlets present will be removed when replenishing the acid solutions.At this stage, the lab will perform a thorough visual inspection of the collagen quality.Spongy bones like ball and sockets, vertebra, and the like do not tend to preserve well in harsh conditions and may not yield sufficient collagen for AMS dating.For bird and fish bones, please consult the lab for sufficient sample size.The d13C and d15N ratios are measured using an isotope ratio mass spectrometer (IRMS).
We may not be able to provide d15N measurements for charred or heated bones depending on the sample quality. Beta Analytic also provides %C, %N, and C: N values at no additional cost for non-cremated bones submitted for AMS dating.
However, the material will be listed as “organics” in the final report instead of “bone collagen.” This is due to the requirement by our ISO/IEC 17005 accreditation that if we did not perform the chemical pretreatments and/or extractions, we cannot specifically testify as to the material that was dated because certain steps affecting the sample quality have been outside of our control and scope of our accreditation.
Do not place extracted collagen directly into Ziplock bags.
– It is important to understand the pretreatment applied to samples since they directly affect the final result.
For bones, we provide conventional collagen extraction techniques and subsequent ultrafiltration methods if requested.
If your samples are already powderized, please contact us for discussion.